Generation Sequencing

Generation sequencing, also known as Sanger sequencing, was invented by Frederick Sanger in 1977. It is the earliest gene sequencing technology and is called the gold standard for gene detection. The principle behind Sanger sequencing involves the incorporation of dideoxynucleotides (ddNTP) into the newly synthesized DNA chain. Because the incorporated ddNTP lacks a 3 '-hydroxyl group, it cannot react with the next nucleotide to form a phosphodiester bond, and the DNA synthesis reaction will be terminated. Sanger sequencing consists of a set of four separate reactions, each containing four deoxynucleoside triphosphates dNTPs, which allow normal synthesis of DNA. Four different dideoxynucleoside triphosphates (ddNTPs) are added to each reaction system, and ddNTPs randomly replace the corresponding dNTPs. Since ddNTPs lack the 3 '-OH group required for extension, they cannot continue to extend, so that the extended oligonucleotides are selectively terminated at G, A, T or C. The termination of the nucleotide by the corresponding ddNTP decision, in order to facilitate positioning, the general use of fluorescence or isotope will be labeled, through electrophoresis can be separated from the length of different fragments, and then the end of these fragments of the base detection to obtain the base sequence of the fragment.

Related Product Recommendation

oliv biological blockbuster launched molecular biology grade high quality products, welcome to order!

ATP,100mM Solution

dATP,100mM Solution

< 1 >